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Image Search Results
Journal: Molecular Systems Biology
Article Title: Path‐seq identifies an essential mycolate remodeling program for mycobacterial host adaptation
doi: 10.15252/msb.20188584
Figure Lengend Snippet: Serial 10‐fold dilutions of MTB H37Rv wild type (wt) and MTB with inducible overexpression of Rv0472c were spotted on 7H10 agar plates with or without ATc. Argentation TLC of 14 C‐labeled methyl esters of mycolic acids (MAMEs) obtained from apolar lipids and delipidated cell wall fractions of MSM wt and MSM with inducible overexpression of MSMEG_ 0916. The α, αʹ, epoxy (e), and cyclopropanated α‐ (X 1 ) MAMEs species are labeled. Faster‐migrating species that co‐migrated with α‐MAMEs and accumulate with induced MSMEG_ 0916 overexpression are indicated as X 2 and X 3 . BCG wt and BCG with inducible overexpression of Rv0472c cultures, labeled with 14 C‐acetate, were induced (+ATc) or uninduced (−ATc) for 4 h or 8 h. The total FAMEs and MAMEs were extracted and analyzed by autoradiography–TLC using equal counts (15,000 cpm) for each lane.
Article Snippet:
Techniques: Over Expression, Labeling, Autoradiography
Journal: Molecular Systems Biology
Article Title: Path‐seq identifies an essential mycolate remodeling program for mycobacterial host adaptation
doi: 10.15252/msb.20188584
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Sequencing, Cloning, Protease Inhibitor, Software, Bacteria